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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
15/07/2015 |
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Data da última atualização: |
15/07/2015 |
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Autoria: |
QUEIROZ, A. de A.; SÁ, M. E. L. de; SILVA, H. E. da. |
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Afiliação: |
ADRIANA DE AZEVEDO QUEIROZ; MARIA EUGÊNIA LISEI DE SÁ; HÉLIO EVALDO DA SILVA. |
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Título: |
Avaliação de mudas de macaúba obtidas in vitro provenientes de diferentes acessos. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 5., 2008, Belo Horizonte. [Anais...]. Belo Horizonte: EPAMIG, 2008. |
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Volume: |
v. 5. |
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Páginas: |
p. 3-4. |
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Idioma: |
Português |
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Conteúdo: |
O objetivo deste trabalho foi avaliar a germinação e o desenvolvimento in vitro de plântulas oriundas de diferentes acessos nativos de macaúba. Projeto Fontes alternativas de matéria prima para produção de biocombustíveis. |
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Thesagro: |
Macaúba; Melhoramento genético vegetal. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/126552/1/2203-183.pdf
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Marc: |
LEADER 00820nam a2200181 a 4500
001 2019875
005 2015-07-15
008 2008 bl uuuu u00u1 u #d
100 1 $aQUEIROZ, A. de A.
245 $aAvaliação de mudas de macaúba obtidas in vitro provenientes de diferentes acessos.
260 $aIn: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 5., 2008, Belo Horizonte. [Anais...]. Belo Horizonte: EPAMIG$c2008
300 $ap. 3-4. v. 5.
490 $vv. 5.
520 $aO objetivo deste trabalho foi avaliar a germinação e o desenvolvimento in vitro de plântulas oriundas de diferentes acessos nativos de macaúba. Projeto Fontes alternativas de matéria prima para produção de biocombustíveis.
650 $aMacaúba
650 $aMelhoramento genético vegetal
700 1 $aSÁ, M. E. L. de
700 1 $aSILVA, H. E. da
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Registro original: |
Embrapa Cerrados (CPAC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
25/03/2011 |
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Data da última atualização: |
15/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MOTA, G. B.; BATISTA, R. I. T. P.; SERAPIÃO, R. V.; BOITE, M. C.; VIANA, J. H. M. |
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Afiliação: |
Gustavo Bruno Mota, UFV; RIBRIO IVAN TAVARES PEREIRA BATISTA, UNIVERSIDADE ESTADUAL DO CEARÁ; RAQUEL VARELA SERAPIAO, PESAGRO - RIO; MARIANA CORTES BOITE, UFF; JOAO HENRIQUE MOREIRA VIANA, CNPGL. |
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Título: |
Developmental competence and expression of the MATER and ZAR1 genes in immature bovine oocytes selected by brilliant cresyl blue. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Zygote, v. 18, n. 3, p. 209-216, 2010. |
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DOI: |
https://doi.org/10.1017/S0967199409990219 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this work was to evaluate the selection of immature bovine oocytes by brilliant cresyl blue dye (BCB) and expression of transcripts MATER and ZAR1. Cumulus–oocyte complexes (COCs) from slaughterhouse ovaries were exposed to BCB diluted in mDPBS and incubated for 60 min at 38.5 °C in humidified air. After exposure those COCs were distributed in two groups, according to their cytoplasm colour: BCB+ (coloured cytoplasm) or BCB− (colourless cytoplasm). The control group was submitted to in vitro maturation (IVM) immediately after morphological selection and holding control group COCs were exposed to mDPBS without BCB but in the same incubation conditions of BCB+ and BCB− group. The COCs of all groups were submitted to IVM, in vitro fertilization (IVF) and in vitro culture (IVC). Cleavage rate (72 h post-insemination) was similar between control (65.3%) and BCB+ (64.4%) groups, but greater than (p < 0.05) holding control (49.8%) and BCB− (51.3%) groups. Blastocyst rate (192 h post-insemination) was not different between BCB+ (18.5%) and control (16.3%) groups, but greater (p < 0.05) than BCB− (8.4%) group. No difference was found for blastocyst rate between holding control group (14.2%), control and BCB+ groups. The relative expression of MATER and ZAR1 genes was evaluated by real-time PCR in immature oocytes collected from the control, holding control, BCB+ and BCB− groups. Despite the relative expression of MATER in holding control, BCB+ and BCB− were down regulated in comparison to control group there was no statistical difference (p > 0.05) in the relative expression of MATER and ZAR1 transcripts among groups. The results indicate that the BCB dye detects immature oocyte populations with different developmental competence, although no improvement in in vitro embryo production using oocytes exposed or not to BCB was observed. Development competence of immature oocytes exposed to BCB does not seem to be associated with variations in the expression of MATER and ZAR1 transcripts. MenosThe objective of this work was to evaluate the selection of immature bovine oocytes by brilliant cresyl blue dye (BCB) and expression of transcripts MATER and ZAR1. Cumulus–oocyte complexes (COCs) from slaughterhouse ovaries were exposed to BCB diluted in mDPBS and incubated for 60 min at 38.5 °C in humidified air. After exposure those COCs were distributed in two groups, according to their cytoplasm colour: BCB+ (coloured cytoplasm) or BCB− (colourless cytoplasm). The control group was submitted to in vitro maturation (IVM) immediately after morphological selection and holding control group COCs were exposed to mDPBS without BCB but in the same incubation conditions of BCB+ and BCB− group. The COCs of all groups were submitted to IVM, in vitro fertilization (IVF) and in vitro culture (IVC). Cleavage rate (72 h post-insemination) was similar between control (65.3%) and BCB+ (64.4%) groups, but greater than (p < 0.05) holding control (49.8%) and BCB− (51.3%) groups. Blastocyst rate (192 h post-insemination) was not different between BCB+ (18.5%) and control (16.3%) groups, but greater (p < 0.05) than BCB− (8.4%) group. No difference was found for blastocyst rate between holding control group (14.2%), control and BCB+ groups. The relative expression of MATER and ZAR1 genes was evaluated by real-time PCR in immature oocytes collected from the control, holding control, BCB+ and BCB− groups. Despite the relative expression of MATER in holding control, BCB+ and BCB− were down regu... Mostrar Tudo |
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Palavras-Chave: |
Brilliant cresyl blue. |
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Thesaurus NAL: |
gene expression; in vitro fertilization; oocytes. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02786naa a2200229 a 4500
001 1883077
005 2024-02-15
008 2010 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S0967199409990219$2DOI
100 1 $aMOTA, G. B.
245 $aDevelopmental competence and expression of the MATER and ZAR1 genes in immature bovine oocytes selected by brilliant cresyl blue.$h[electronic resource]
260 $c2010
520 $aThe objective of this work was to evaluate the selection of immature bovine oocytes by brilliant cresyl blue dye (BCB) and expression of transcripts MATER and ZAR1. Cumulus–oocyte complexes (COCs) from slaughterhouse ovaries were exposed to BCB diluted in mDPBS and incubated for 60 min at 38.5 °C in humidified air. After exposure those COCs were distributed in two groups, according to their cytoplasm colour: BCB+ (coloured cytoplasm) or BCB− (colourless cytoplasm). The control group was submitted to in vitro maturation (IVM) immediately after morphological selection and holding control group COCs were exposed to mDPBS without BCB but in the same incubation conditions of BCB+ and BCB− group. The COCs of all groups were submitted to IVM, in vitro fertilization (IVF) and in vitro culture (IVC). Cleavage rate (72 h post-insemination) was similar between control (65.3%) and BCB+ (64.4%) groups, but greater than (p < 0.05) holding control (49.8%) and BCB− (51.3%) groups. Blastocyst rate (192 h post-insemination) was not different between BCB+ (18.5%) and control (16.3%) groups, but greater (p < 0.05) than BCB− (8.4%) group. No difference was found for blastocyst rate between holding control group (14.2%), control and BCB+ groups. The relative expression of MATER and ZAR1 genes was evaluated by real-time PCR in immature oocytes collected from the control, holding control, BCB+ and BCB− groups. Despite the relative expression of MATER in holding control, BCB+ and BCB− were down regulated in comparison to control group there was no statistical difference (p > 0.05) in the relative expression of MATER and ZAR1 transcripts among groups. The results indicate that the BCB dye detects immature oocyte populations with different developmental competence, although no improvement in in vitro embryo production using oocytes exposed or not to BCB was observed. Development competence of immature oocytes exposed to BCB does not seem to be associated with variations in the expression of MATER and ZAR1 transcripts.
650 $agene expression
650 $ain vitro fertilization
650 $aoocytes
653 $aBrilliant cresyl blue
700 1 $aBATISTA, R. I. T. P.
700 1 $aSERAPIÃO, R. V.
700 1 $aBOITE, M. C.
700 1 $aVIANA, J. H. M.
773 $tZygote$gv. 18, n. 3, p. 209-216, 2010.
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